Module Details

Module Code: BIOT6002
Title: Immunoanalysis
Long Title: Immunoanalysis
NFQ Level: Fundamental
Valid From: Semester 1 - 2017/18 ( September 2017 )
Duration: 1 Semester
Credits: 5
Field of Study: 4218 - Biotechnology
Module Delivered in: 7 programme(s)
Module Description: This module introduces the student to modern immuno-analytical techniques. The concepts and principles of immunochemistry are introduced, as well as the basic principles of biostatistics and quality assurance in the bioanalytical laboratory.
 
Learning Outcomes
On successful completion of this module the learner will be able to:
# Learning Outcome Description
LO1 Explain and outline the basic principles of immunochemistry
LO2 Describe and explain the principles of standardisation,optimisation and validation of immunoassay systems
LO3 Outline the elements of quality control and quality assurance in a bioanalytical laboratory
LO4 Use biostatistics to analyse data and solve quantitative problems
LO5 Demonstrate and employ a range of immuno-analytical laboratory skills
Dependencies
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).

Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed
Requirements

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.

No requirements listed
 
Indicative Content
Introduction to Immunochemistry
Structures of antibodies, antibody/antigen interaction, immunoprecipitation, immunodiffusion and immunoelectrophoresis techniques, immunoaffinity chromatography, introduction to immunoassay classification
Immunoassay Techniques
Immunoassay principles & techniques, Particle immunoassays & automation, Optimisation & method validation
Quality assurance and biostatistics
Introduction to the principles of standardisation & IQC in immunoassay, internal and external QA schemes, basic statistical methods & data handling, Control charts and acceptance criteria, reliability and sources of error, Principles of Good Laboratory Practice
Laboratory practice & data analysis
Pipetting skills, immunoprecipitation, optimisation of enzyme linked immunosorbent assay (ELISA), statistical data analysis for validation study
Module Content & Assessment
Assessment Breakdown%
Coursework40.00%
End of Module Formal Examination60.00%

Assessments

Coursework
Assessment Type Multiple Choice Questions % of Total Mark 10
Timing Week 7 Learning Outcomes 1
Assessment Description
Assessment of basic principles of immunochemistry
Assessment Type Practical/Skills Evaluation % of Total Mark 30
Timing Every Second Week Learning Outcomes 4,5
Assessment Description
Practical reports
End of Module Formal Examination
Assessment Type Formal Exam % of Total Mark 60
Timing End-of-Semester Learning Outcomes 1,2,3
Assessment Description
End-of-Semester Final Examination
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The University reserves the right to alter the nature and timings of assessment

 

Module Workload

Workload: Full Time
Workload Type Contact Type Workload Description Frequency Average Weekly Learner Workload Hours
Lecture Contact Lecture Every Week 2.00 2
Lab Contact Lab Every Second Week 1.00 2
Independent & Directed Learning (Non-contact) Non Contact Independent & directed learning Every Week 4.00 4
Total Hours 8.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 3.00
This module has no Part Time workload.
 
Module Resources
Recommended Book Resources
  • James P. Gosling. (2006), Immunoassays, Oxford University Press, Oxford, [ISBN: 0-19-963711-3].
  • David J Holme & Hazel Peck. (2000), Analytical Biochemistry, 3rd. John Wiley & Sons Ltd, [ISBN: 9780582294387].
  • Elizabeth Prichard, Vicki Barwick. (2007), Quality assurance in analytical chemistry, John Wiley & Sons, [ISBN: 0470012048].
Supplementary Book Resources
  • David Wild. (2013), The Immunoassay Handbook, 4th. Elsevier Science & Technology, [ISBN: 0080970370].
This module does not have any article/paper resources
This module does not have any other resources
 
Module Delivered in
Programme Code Programme Semester Delivery
CR_SHERB_8 Bachelor of Science (Honours) in Herbal Science 3 Mandatory
CR_SNHSC_8 Bachelor of Science (Honours) in Nutrition and Health Science 3 Mandatory
CR_SPHBI_8 Bachelor of Science (Honours) in Pharmaceutical Biotechnology 3 Mandatory
CR_SBIBI_7 Bachelor of Science in Applied Biosciences and Biotechnology 3 Mandatory
CR_SFSTE_7 Bachelor of Science in Food and Health Science 3 Mandatory
CR_SCEBS_8 Common Entry Biological Sciences 3 Mandatory
CR_SBIOS_6 Higher Certificate in Science in Applied Biosciences 3 Mandatory