Module Details
| Module Code: |
BIOL6017 |
| Title: |
Metabolic Biochemistry
|
|
Long Title:
|
Metabolic Biochemistry
|
| NFQ Level: |
Fundamental |
| Valid From: |
Semester 1 - 2017/18 ( September 2017 ) |
| Field of Study: |
4211 - Biochemistry & Cell Biology
|
| Module Description: |
This module teaches the principles of enzyme catalysis, inhibition and regulation, leading on to a study of selected metabolic pathways and their integration and control.
|
| Learning Outcomes |
| On successful completion of this module the learner will be able to: |
| # |
Learning Outcome Description |
| LO1 |
Explain the principles of enzyme catalysis, inhibition and regulation. |
| LO2 |
Design and optimise assays to monitor enzyme reactions and assess reaction rates. |
| LO3 |
Outline selected metabolic pathways and describe the principles that govern their regulation |
| LO4 |
Perform laboratory experiments to demonstrate enzyme action and quantify reaction rate constants. |
| LO5 |
Write scientific reports to analyse and interpret experimental data |
| Dependencies |
Module Recommendations
This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).
|
| 12751 |
BIOL6017 |
Metabolic Biochemistry |
Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
|
| No incompatible modules listed |
Co-requisite Modules
|
| No Co-requisite modules listed |
Requirements
This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.
|
| No requirements listed |
| Indicative Content |
|
Enzymes
Biological functions of enzymes / Energy of activation and catalysis / Classification of enzymes / Enzyme-substrate complex / Kinetics (Michaelis-Menton, Km, Vmax, Ki, Lineweaver Burk) / Temperature and pH effects / Regulation of enzyme activity / Enzyme inhibition / Units of enzyme activity / Introduction to enzyme assay design (monitoring assays, coupling reactions, fixed time assays and kinetic assays).
|
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Cellular energetics and ATP
Biological redox reactions, reduction potential/energy coupling reactions, ATP hydrolysis.
|
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Carbohydrate metabolism
Overview of relevant metabolic pathways (glycolysis, citric acid cycle, electron transport chain) / substrate level and oxidative phosphorylation / metabolic regulation and control points.
|
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Gluconeogenesis
Overview of metabolic pathway / Cori cycle / metabolic control points / hormonal regulation
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Lipid metabolism
Overview of Fatty acid metabolism / beta oxidation / metabolic regulation.
|
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Urea cycle
Amino acid metabolism / Ammonia formation / overview of the urea cycle / metabolic regulation.
|
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Practical skills
Methods for quantitation of biomolecules / determination of Km and Vmax using fixed time and continuous monitoring methods / effect of temperature, pH and reactant concentrations on enzyme reaction rates / estimation of precision and accuracy of experimental results.
|
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Module Content & Assessment
|
| Assessment Breakdown | % |
|---|
| Coursework | 100.00% |
Assessments
| No End of Module Formal Examination |
| Reassessment Requirement |
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.
|
The University reserves the right to alter the nature and timings of assessment
Module Workload
| Workload: Full Time |
| Workload Type |
Contact Type |
Workload Description |
Frequency |
Average Weekly Learner Workload |
Hours |
| Lecture |
Contact |
class-based instruction |
Every Week |
2.00 |
2 |
| Lab |
Contact |
practical laboratory instruction |
Every Week |
2.00 |
2 |
| Independent & Directed Learning (Non-contact) |
Non Contact |
study of lecture notes |
Every Week |
2.00 |
2 |
| Independent & Directed Learning (Non-contact) |
Non Contact |
report preparation, data handling exercises |
Every Week |
1.00 |
1 |
| Total Hours |
7.00 |
| Total Weekly Learner Workload |
7.00 |
| Total Weekly Contact Hours |
4.00 |
| This module has no Part Time workload. |
|
Module Resources
|
| Recommended Book Resources |
|---|
-
Nelson, D.L. & Cox, M. M.. (2017), Lehninger principles of biochemistry, 7th. Freeman and Sapling, [ISBN: 9781464126116].
| | Supplementary Book Resources |
|---|
-
Berg, J.M., Tymoczko, J.L., Gatto, G.J. & Stryer, L.. (2015), Biochemistry, 8th. W.H. Freeman, [ISBN: 9781464126109].
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Moorthy, K.. (2007), Fundamentals of Biochemical Calculations, 2nd. CRC Press, [ISBN: 1420053574].
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Dwivedi, Neeraja, Dwivedi, Prakash. (2009), Comprehensive Biochemistry, Ebook, Pragati Prakashan, [ISBN: 9789350434536].
| | This module does not have any article/paper resources |
|---|
| Other Resources |
|---|
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Website, Interactive Concepts in Biochemisrty, Wiley,
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Website, Kevin Ahern and Indira Rajagopal. Biochemistry Free & Easy,
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