Module Details

Module Code: BIOL8018
Title: Structural Biochemistry UCC
Long Title: Structural Biochemistry UCC
NFQ Level: Advanced
Valid From: Semester 1 - 2017/18 ( September 2017 )
Duration: 1 Semester
Credits: 5
Field of Study: 4211 - Biochemistry & Cell Biology
Module Delivered in: 1 programme(s)
Module Description: Structural studies of proteins and nucleic acids.
 
Learning Outcomes
On successful completion of this module the learner will be able to:
# Learning Outcome Description
LO1 Describe the key structural features of the main informational biomacromolecules
LO2 Outline how proteins and DNA plasmids are routinely purified
LO3 Discuss the physical and chemical basis of chromatography
LO4 Describe how important sequence is to function
LO5 Appreciate the origin and use of sequence data
LO6 Describe the basis and application of electrophoresis methods in Biochemistry
Dependencies
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).
Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed
Requirements

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.
BC2001
 
Indicative Content
Amino acids, peptides and proteins
Chemistry, structure and clasification of amino acids, The peptide bond, Peptide structure and bioactive peptides, The protein structural heirarchy with examples.
Isolation of informational biomacromolecules-
Precipitation of proteins,Chromatography o f proteins including mode (ion exchange, size exclusion and affinity) and resolution (HPLC and FPLC), Protein purification tables, Alkali-phenol lysis for preparation of plasmid DNA, Isolation of mRNA by oligo dT-cellulose.
Sequencing of informational biomacromolecules
Direct sequencing by Edman degradation,Sequencing of proteins by mass spectrometry, Sanger sequencing of DNA, Sequence databases.
Solid Phase methods
Merrifield peptide synthesis, Synthesis of oligonulceotides.
Electrophoresis
Native electrophoresis, SDS PAGE, Isoelectric focusing, 2D SDS PAGE, Blotting methods, Electroelution.
Enzymes Purification of organelles, membranes and membrane proteins from cells
Basis of catalysis, Michaelis-Menten equation, Kinetic parameters, Enzyme inhibition. Allosterism and metabolic control
Module Content & Assessment
Assessment Breakdown%
Coursework40.00%
End of Module Formal Examination60.00%

Assessments

Coursework
Assessment Type Practical/Skills Evaluation % of Total Mark 10
Timing Every Week Learning Outcomes 3,4,6
Assessment Description
n/a
Assessment Type Multiple Choice Questions % of Total Mark 30
Timing Sem End Learning Outcomes 1,2,3,4,5,6
Assessment Description
n/a
End of Module Formal Examination
Assessment Type Formal Exam % of Total Mark 60
Timing End-of-Semester Learning Outcomes 1,2,3,4,5,6
Assessment Description
End-of-Semester Final Examination

The University reserves the right to alter the nature and timings of assessment

 

Module Workload

Workload: Full Time
Workload Type Contact Type Workload Description Frequency Average Weekly Learner Workload Hours
Lecture Contact For 6 weeks Every Week 2.00 2
Lab Contact For 3 weeks Every Week 2.00 2
Independent & Directed Learning (Non-contact) Non Contact For 6 weeks Every Week 3.00 3
Total Hours 7.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 4.00
This module has no Part Time workload.
 
Module Resources
Recommended Book Resources
  • Lehninger, Cox & Nelson. Principles of Biochemistry, 4th. Worth.
  • Reed, Holmes, Weyers & Jones. Practical Skills in Biomolecular Sciences, Longman.
This module does not have any article/paper resources
This module does not have any other resources
 
Module Delivered in
Programme Code Programme Semester Delivery
CR_SBISC_8 Bachelor of Science (Honours) in Biomedical Science 5 Mandatory