Module Details

Module Code: CHEA8001
Title: Advanced Chromatography
Long Title: Advanced Chromatography
NFQ Level: Advanced
Valid From: Semester 1 - 2019/20 ( September 2019 )
Duration: 1 Semester
Credits: 5
Field of Study: 4424 - Analytical Chemistry
Module Delivered in: 1 programme(s)
Module Description: This module presents the main approaches to chromatographic method development, optimisation and analysis.
 
Learning Outcomes
On successful completion of this module the learner will be able to:
# Learning Outcome Description
LO1 Describe in detail the theoretic principles governing chromatographic separations
LO2 Display a comprehensive knowledge of the instrumentation and operation of chromatographic equipment
LO3 Perform a strategic assessment of the analytical problem and hence design appropriate methods to answer the analytical question and execute a systematic approach to trouble-shooting problems in chromatographic separations
LO4 Describe various hyphenated techniques and understand their capabilities and limitations in answering the analytical question
LO5 Propose the essential sample preparation requirements for trace analytes in complex matrices prior to chromatographic analysis
Dependencies
Module Recommendations

This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named MTU module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).
Incompatible Modules
These are modules which have learning outcomes that are too similar to the learning outcomes of this module. You may not earn additional credit for the same learning and therefore you may not enrol in this module if you have successfully completed any modules in the incompatible list.
No incompatible modules listed
Co-requisite Modules
No Co-requisite modules listed
Requirements

This is prior learning (or a practical skill) that is mandatory before enrolment in this module is allowed. You may not enrol on this module if you have not acquired the learning specified in this section.
No requirements listed
 
Indicative Content
Chromatography Theory
Separation parameters. Optimisation Parameters. Sorption mechanisms, plate theory versus rate theory, problem solving.
Liquid solid chromatography
Liquid chromatography (LC) instrumentation. LC mobile phase optimisation. LC column characteristics. Stationary phase technology. Peak shape optimisation,Van Deemter Equation. Multidimensional chromatography. Ion Chromatography. Ultra Performance LC (UPLC) Derivatisation methods for LC.
Gas chromatography (GC)
GC instrumentation. GC column design and stationary phases. Sampling and injection methods for GC. Derivatisation methods in GC analysis. Solid Phase Micro Extraction
Chromatography-Mass Spectrometry
Liquid chromatography-mass spectrometry (LC-MS and LC-MS/MS) instrumentation: Principles, operation, and applications. Gas chromatography-mass spectrometry (GC-MS and GC-MS/MS)instrumentation: Principles, instrumentation and operation. Matrix Effects and Sample clean-up by Solid Phase Extraction. Solid Phase Micro Extraction (SPME) sampling.
Supercritical Fluid Chromatography (SFC)
Principles, operation and applications of SFC
Preparative Chromatography
Theory, Principles and Applications. LC, Size Exclusion Chromatography and Chiral Chromatography. Large scale GC and TLC.
Planar Chromatography
Thin Layer Chromatography (TLC), principles and applications, 2-dimensional TLC, detection systems.
Module Content & Assessment
Assessment Breakdown%
Coursework30.00%
End of Module Formal Examination70.00%

Assessments

Coursework
Assessment Type Presentation % of Total Mark 15
Timing Sem End Learning Outcomes 2,3,5
Assessment Description
Selected applications of chromatographic methods.
Assessment Type Short Answer Questions % of Total Mark 15
Timing Week 7 Learning Outcomes 1,2,4
Assessment Description
Theoretical principles of LC and GC
End of Module Formal Examination
Assessment Type Formal Exam % of Total Mark 70
Timing End-of-Semester Learning Outcomes 1,2,3,4,5
Assessment Description
End-of-Semester Final Examination
Reassessment Requirement
Repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

The University reserves the right to alter the nature and timings of assessment

 

Module Workload

Workload: Full Time
Workload Type Contact Type Workload Description Frequency Average Weekly Learner Workload Hours
Lecture Contact Advanced Chromatography Every Week 3.00 3
Independent & Directed Learning (Non-contact) Non Contact Personal study Every Week 4.00 4
Total Hours 7.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 3.00
Workload: Part Time
Workload Type Contact Type Workload Description Frequency Average Weekly Learner Workload Hours
Lecture Contact Advanced Chromatography Every Week 3.00 3
Independent & Directed Learning (Non-contact) Non Contact Personal Study Every Week 4.00 4
Total Hours 7.00
Total Weekly Learner Workload 7.00
Total Weekly Contact Hours 3.00
 
Module Resources
Recommended Book Resources
  • Veronika R. Meyer,. (2010), Practical High-Performance Liquid Chromatography, 5th. Wiley-Blackwell, N.J., U.S., [ISBN: 978-0470682180].
Supplementary Book Resources
  • Robert E. Ardrey. (2009), Liquid chromatography-mass spectrometry (An Introduction), J. Wiley, N.Y., U.S., [ISBN: 0471498017].
  • David O. Sparkman. (2011), Gas Chromatography and Mass Spectrometry, 2nd. Elsevier, Oxford, U.K., [ISBN: 978-0123736284].
  • Gerard Rosse. (2018), Supercritical fluid Chromatography, de Gruyter, Germany, [ISBN: 310500752].
This module does not have any article/paper resources
This module does not have any other resources
 
Module Delivered in
Programme Code Programme Semester Delivery
CR_SCHQA_8 Bachelor of Science (Honours) in Analytical Chemistry with Quality Assurance 7 Mandatory